Multilocus genotype summary

Summary of multilocus genotypes of Phytophthora infestans collected in the US and Canada, 2002-2009 (from Hu et al., Plant Dis. 2012 and Fry et al., APSnet Features 2012)

Genotypea Host Mating type Allozyme genotypeb Sensitivity to mefenoxamc mtDNA haplotyped RG57 RFLPe
Gpi Pep
US-8f Potato A2 100/111/122 100/100 I/R Ia 1,5,10,13,14,16,20,21,23,24,25
US-11 Potato/Tomato A1 100/111 100/100 R Ia 1,3,5,6,7,10,13,14,16,18,20,21,24,25
US-20 Tomato A2 100/100 100/100 I/R Ia 1,3,5,7,10,13,14,16,18,20,21,24,25
US-21 Tomato A2 100/122 100/100 S/I/R Ia 1,5,10,13,14,18,20,21,24,25
US-22g Potato/Tomato A2 100/122 100/100 S/I Ia 1,5,13,14,16,20,21,24,25
US-23 Potato/Tomato A1 100/100 100/100 S/I Ia 1,2,5,6,10,13,14,17,20,21,24,24a,25
US-24 Potato A1 100/100/111 100/100 I Ia 1,3,5,7,10,13,14,16,20,21,23,24,25

aUS-22 caused the majority of potato and tomato late blight outbreaks in the Eastern US in 2009.

bAllozyme genotyping done using methods of Goodwin et al. 1995.

S= mefenoxam sensitive, I= intermediate, R=resistant.

dmtDNA haplotype determined by the methods of Griffith and Shaw 1998.

eDNA fingerprinting by RFLP by methods of Goodwin et al. 1992.

fThe SSR alleles for the US-8 genotype are: 163/165, 176/179, 213/226, 155/155, 166/170, 190/190, 257/257, 280/280, 106/110,173/177,
202/202 for the Pi02, Pi89, Pi4B, PiG11, Pi04, Pi70, Pi56, Pi63, D13, Pi16 and Pi33 loci, respectively by the methods of Lees et al. 2006.

gSSR alleles for the US-22 genotype were: 163/165, 176/179, 213/213, 131/155, 166/170, 190/193, 257/257,280/280, 177/177, 202/205
for the Pi02, Pi89, Pi4B, PiG11, Pi04, Pi70, Pi56, Pi63, Pi16 and Pi33 loci, respectively by the methods of Lees et al. 2006.